About the position

The position focuses on construct development and performing genomic DNA extractions, PCR, Southern blotting, qRT-PCR, sequencing, mutation detection, and maintaining bacterial cultures. The successful candidate will work closely with a multidisciplinary team of scientists and contribute to ongoing research in plant genome editing and trait enhancement.

Key Responsibilities

• Develop genome editing and RNAi constructs.
• Perform genomic DNA extraction from various transgenic and gene edited events of date palm, sugarcane or any other crops for molecular analysis.
• Conduct PCR (Polymerase Chain Reaction) and qRT-PCR (quantitative Real-Time PCR) to assess gene expression and mutation verification.
• Perform mutation detection assays, including T7 endonuclease and band-shift analysis.
• Carry out Southern blot analysis for gene copy number determination and transgene integration.
• Sequence analysis for detection and validation of gene edits using techniques such as Sanger or next-generation sequencing.
• Maintain and propagate bacterial cultures for plasmid DNA preparations.
• Contribute to the maintenance of gene-edited plant lines in the lab and greenhouse.
• Ensure accurate data collection, analysis, and record-keeping for experimental results.
• Assist in preparing reports, presentations, and manuscripts related to the research findings.
• Collaborate with other team members and provide technical support in molecular techniques.
• Perform any other duty assigned

Requirements

• Bachelor’s degree or diploma in molecular biology, Biotechnology, Genetics, or a related field.
• Relevant professional certification.
• Two years relevant experience with degree or four years’ relevant experience with diploma. Experience with in silico gene cloning tools such as Benchling and SnapGene
• Hands-on experience with molecular techniques such as DNA extraction, PCR, qRT-PCR, and sequencing.
• Familiarity with Southern blotting and mutation detection assays is a plus.
• Ability to maintain detailed lab records and follow protocols with precision.
• Strong organizational and time management skills.
• Excellent interpersonal and communication skills for working in a collaborative research environment.

• Develop genome editing and RNAi constructs.
• Perform genomic DNA extraction from various transgenic and gene edited events of date palm, sugarcane or any other crops for molecular analysis.
• Conduct PCR (Polymerase Chain Reaction) and qRT-PCR (quantitative Real-Time PCR) to assess gene expression and mutation verification.
• Perform mutation detection assays, including T7 endonuclease and band-shift analysis.
• Carry out Southern blot analysis for gene copy number determination and transgene integration.
• Sequence analysis for detection and validation of gene edits using techniques such as Sanger or next-generation sequencing.
• Maintain and propagate bacterial cultures for plasmid DNA preparations.
• Contribute to the maintenance of gene-edited plant lines in the lab and greenhouse.
• Ensure accurate data collection, analysis, and record-keeping for experimental results.
• Assist in preparing reports, presentations, and manuscripts related to the research findings.
• Collaborate with other team members and provide technical support in molecular techniques.
• Perform any other duty assigned

• In silico gene cloning tools such as Benchling and SnapGene
• DNA extraction
• PCR
• qRT-PCR
• Sequencing
• Southern blotting
• Mutation detection assays
• Data collection and analysis
• Record-keeping
• Organizational skills
• Time management skills
• Interpersonal skills
• Communication skills

• Bachelor’s degree or diploma in molecular biology, Biotechnology, Genetics, or a related field.
• Relevant professional certification.
• Two years relevant experience with degree or four years’ relevant experience with diploma.